PhytophthoraV1, Main, Exploration, bibRecord, 000D56

The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.

Identifieur interne : 000D56 ( Main/Exploration ); précédent : 000D55; suivant : 000D57

The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.

Auteurs : Tassanee Lerksuthirat [Thaïlande] ; Tassanee Lohnoo [Thaïlande] ; Ruchuros Inkomlue [Thaïlande] ; Thidarat Rujirawat [Thaïlande] ; Wanta Yingyong [Thaïlande] ; Rommanee Khositnithikul [Thaïlande] ; Narumon Phaonakrop [Thaïlande] ; Sittiruk Roytrakul [Thaïlande] ; Thomas D. Sullivan [États-Unis] ; Theerapong Krajaejun [Thaïlande]

Source :

PloS one [ 1932-6203 ] ; 2015.

RBID : pubmed:25793767

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Antibody Formation (immunology), Base Sequence (MeSH), Cloning, Molecular (MeSH), Genome (MeSH), Glycoproteins (chemistry), Glycoproteins (genetics), Glycoproteins (immunology), Glycoproteins (metabolism), Glycosylation (MeSH), Host-Pathogen Interactions (immunology), Humans (MeSH), Immune Evasion (MeSH), Mass Spectrometry (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Promoter Regions, Genetic (genetics), Proteome (metabolism), Pythium (genetics), Pythium (immunology), Recombinant Proteins (metabolism), Sequence Alignment (MeSH), Sequence Homology, Amino Acid (MeSH), Transcriptome (genetics).
MESH :
- chemical , chemistry : Glycoproteins.
- chemical , genetics : Glycoproteins.
- genetics : Promoter Regions, Genetic, Pythium, Transcriptome.
- immunology : Antibody Formation, Glycoproteins, Host-Pathogen Interactions, Pythium.
- chemical , metabolism : Glycoproteins, Proteome, Recombinant Proteins.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genome, Glycosylation, Humans, Immune Evasion, Mass Spectrometry, Molecular Sequence Data, Phylogeny, Sequence Alignment, Sequence Homology, Amino Acid.

Abstract

Pythium insidiosum is a unique oomycete that can infect humans and animals. Patients with a P. insidiosum infection (pythiosis) have high rates of morbidity and mortality. The pathogen resists conventional antifungal drugs. Information on the biology and pathogenesis of P. insidiosum is limited. Many pathogens secrete proteins, known as effectors, which can affect the host response and promote the infection process. Elicitins are secretory proteins and are found only in the oomycetes, primarily in Phytophthora and Pythium species. In plant-pathogenic oomycetes, elicitins function as pathogen-associated molecular pattern molecules, sterol carriers, and plant defense stimulators. Recently, we reported a number of elicitin-encoding genes from the P. insidiosum transcriptome. The function of elicitins during human infections is unknown. One of the P. insidiosum elicitin-encoding genes, ELI025, is highly expressed and up-regulated at body temperature. This study aims to characterize the biochemical, immunological, and genetic properties of the elicitin protein, ELI025. A 12.4-kDa recombinant ELI025 protein (rELI025) was expressed in Escherichia coli. Rabbit anti-rELI025 antibodies reacted strongly with the native ELI025 in P. insidiosum's culture medium. The detected ELI025 had two isoforms: glycosylated and non-glycosylated. ELI025 was not immunoreactive with sera from pythiosis patients. The region near the transcriptional start site of ELI025 contained conserved oomycete core promoter elements. In conclusion, ELI025 is a small, abundant, secreted glycoprotein that evades host antibody responses. ELI025 is a promising candidate for development of diagnostic and therapeutic targets for pythiosis.

DOI: 10.1371/journal.pone.0118547
PubMed: 25793767
PubMed Central: PMC4368664

Affiliations:

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<term>Antibody Formation (immunology)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Genome (MeSH)</term>
<term>Glycoproteins (chemistry)</term>
<term>Glycoproteins (genetics)</term>
<term>Glycoproteins (immunology)</term>
<term>Glycoproteins (metabolism)</term>
<term>Glycosylation (MeSH)</term>
<term>Host-Pathogen Interactions (immunology)</term>
<term>Humans (MeSH)</term>
<term>Immune Evasion (MeSH)</term>
<term>Mass Spectrometry (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Promoter Regions, Genetic (genetics)</term>
<term>Proteome (metabolism)</term>
<term>Pythium (genetics)</term>
<term>Pythium (immunology)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Transcriptome (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Glycoprotéines (composition chimique)</term>
<term>Glycoprotéines (génétique)</term>
<term>Glycoprotéines (immunologie)</term>
<term>Glycoprotéines (métabolisme)</term>
<term>Glycosylation (MeSH)</term>
<term>Génome (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Interactions hôte-pathogène (immunologie)</term>
<term>Phylogenèse (MeSH)</term>
<term>Production d'anticorps (immunologie)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Protéome (métabolisme)</term>
<term>Pythium (génétique)</term>
<term>Pythium (immunologie)</term>
<term>Régions promotrices (génétique) (génétique)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Spectrométrie de masse (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Transcriptome (génétique)</term>
<term>Échappement immunitaire (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glycoproteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycoproteins</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glycoprotéines</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Promoter Regions, Genetic</term>
<term>Pythium</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines</term>
<term>Pythium</term>
<term>Régions promotrices (génétique)</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines</term>
<term>Interactions hôte-pathogène</term>
<term>Production d'anticorps</term>
<term>Pythium</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Formation</term>
<term>Glycoproteins</term>
<term>Host-Pathogen Interactions</term>
<term>Pythium</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycoproteins</term>
<term>Proteome</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines</term>
<term>Protéines recombinantes</term>
<term>Protéome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Genome</term>
<term>Glycosylation</term>
<term>Humans</term>
<term>Immune Evasion</term>
<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Sequence Alignment</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Glycosylation</term>
<term>Génome</term>
<term>Humains</term>
<term>Phylogenèse</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Spectrométrie de masse</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Échappement immunitaire</term>
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<front><div type="abstract" xml:lang="en">Pythium insidiosum is a unique oomycete that can infect humans and animals. Patients with a P. insidiosum infection (pythiosis) have high rates of morbidity and mortality. The pathogen resists conventional antifungal drugs. Information on the biology and pathogenesis of P. insidiosum is limited. Many pathogens secrete proteins, known as effectors, which can affect the host response and promote the infection process. Elicitins are secretory proteins and are found only in the oomycetes, primarily in Phytophthora and Pythium species. In plant-pathogenic oomycetes, elicitins function as pathogen-associated molecular pattern molecules, sterol carriers, and plant defense stimulators. Recently, we reported a number of elicitin-encoding genes from the P. insidiosum transcriptome. The function of elicitins during human infections is unknown. One of the P. insidiosum elicitin-encoding genes, ELI025, is highly expressed and up-regulated at body temperature. This study aims to characterize the biochemical, immunological, and genetic properties of the elicitin protein, ELI025. A 12.4-kDa recombinant ELI025 protein (rELI025) was expressed in Escherichia coli. Rabbit anti-rELI025 antibodies reacted strongly with the native ELI025 in P. insidiosum's culture medium. The detected ELI025 had two isoforms: glycosylated and non-glycosylated. ELI025 was not immunoreactive with sera from pythiosis patients. The region near the transcriptional start site of ELI025 contained conserved oomycete core promoter elements. In conclusion, ELI025 is a small, abundant, secreted glycoprotein that evades host antibody responses. ELI025 is a promising candidate for development of diagnostic and therapeutic targets for pythiosis. </div>
</front>
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<ArticleTitle>The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0118547</ELocationID>
<Abstract><AbstractText>Pythium insidiosum is a unique oomycete that can infect humans and animals. Patients with a P. insidiosum infection (pythiosis) have high rates of morbidity and mortality. The pathogen resists conventional antifungal drugs. Information on the biology and pathogenesis of P. insidiosum is limited. Many pathogens secrete proteins, known as effectors, which can affect the host response and promote the infection process. Elicitins are secretory proteins and are found only in the oomycetes, primarily in Phytophthora and Pythium species. In plant-pathogenic oomycetes, elicitins function as pathogen-associated molecular pattern molecules, sterol carriers, and plant defense stimulators. Recently, we reported a number of elicitin-encoding genes from the P. insidiosum transcriptome. The function of elicitins during human infections is unknown. One of the P. insidiosum elicitin-encoding genes, ELI025, is highly expressed and up-regulated at body temperature. This study aims to characterize the biochemical, immunological, and genetic properties of the elicitin protein, ELI025. A 12.4-kDa recombinant ELI025 protein (rELI025) was expressed in Escherichia coli. Rabbit anti-rELI025 antibodies reacted strongly with the native ELI025 in P. insidiosum's culture medium. The detected ELI025 had two isoforms: glycosylated and non-glycosylated. ELI025 was not immunoreactive with sera from pythiosis patients. The region near the transcriptional start site of ELI025 contained conserved oomycete core promoter elements. In conclusion, ELI025 is a small, abundant, secreted glycoprotein that evades host antibody responses. ELI025 is a promising candidate for development of diagnostic and therapeutic targets for pythiosis. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lerksuthirat</LastName>
<ForeName>Tassanee</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Molecular Medicine Program, Multidisciplinary Unit, Faculty of Science, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lohnoo</LastName>
<ForeName>Tassanee</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Inkomlue</LastName>
<ForeName>Ruchuros</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rujirawat</LastName>
<ForeName>Thidarat</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Molecular Medicine Program, Multidisciplinary Unit, Faculty of Science, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yingyong</LastName>
<ForeName>Wanta</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Khositnithikul</LastName>
<ForeName>Rommanee</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Phaonakrop</LastName>
<ForeName>Narumon</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Proteomics Research Laboratory, Genome Institute, National Science and Technology Development Agency, Pathum Thani, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Roytrakul</LastName>
<ForeName>Sittiruk</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Proteomics Research Laboratory, Genome Institute, National Science and Technology Development Agency, Pathum Thani, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sullivan</LastName>
<ForeName>Thomas D</ForeName>
<Initials>TD</Initials>
<AffiliationInfo><Affiliation>Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Krajaejun</LastName>
<ForeName>Theerapong</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName>
<AccessionNumberList><AccessionNumber>AB971191</AccessionNumber>
<AccessionNumber>AB971192</AccessionNumber>
<AccessionNumber>AB971193</AccessionNumber>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>03</Month>
<Day>20</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D020543">Proteome</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016678" MajorTopicYN="N">Genome</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057131" MajorTopicYN="N">Immune Evasion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011775" MajorTopicYN="N">Pythium</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<Day>20</Day>
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<ArticleIdList><ArticleId IdType="pubmed">25793767</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0118547</ArticleId>
<ArticleId IdType="pii">PONE-D-14-41687</ArticleId>
<ArticleId IdType="pmc">PMC4368664</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>PLoS Genet. 2013 Jun;9(6):e1003272</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23785293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2013;9(7):e1003518</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23935490</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2012;3:686</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22353717</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2014 Jan;42(Database issue):D705-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24194595</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4352-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21368207</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2005 Mar;11(3):479-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15757572</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2001 Jan 19;305(3):567-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11152613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Biol. 2011 Jul;115(7):683-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21724174</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Sep 1;313(5791):1261-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16946064</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 1987 Jul;4(4):406-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3447015</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2006;1(6):2856-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17406544</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Mol Biol Rev. 2010 Dec;74(4):479-503</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21119014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycopathologia. 1986 May;94(2):123-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3088454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Microbiol Rev. 2010 Oct;23(4):713-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20930071</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Genet Genomics. 2005 Mar;273(1):20-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15702346</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Microbiol. 2010 Nov 20;146(1-2):1-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20800978</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2012;4(1):e00428-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23269827</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2001 Sep;12(9):2825-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11553720</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Assoc Thai. 2014 Mar;97(3):342-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25123015</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2012 Oct;25(10):1350-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22712506</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Microbiol. 2012 Dec;15(6):685-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23177095</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2011;8(10):785-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2006 Feb;23(2):338-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16237208</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 1997 Oct 20;416(2):190-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9369212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genet. 2003 Mar;42(6):344-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12612808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 1999;112:531-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10027275</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Comput Appl Biosci. 1992 Jun;8(3):275-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1633570</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 1999 Jun;8(6):1191-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10386869</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2011 Jul;9(7):e1001094</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21750662</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genet. 1997 Mar;31(3):272-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9065391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 1998 Apr 7;245(1):133-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9535796</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Vaccine Immunol. 2009 Apr;16(4):506-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19225072</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008;3(3):e1723</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18320043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2004 Oct 1;306(5693):79-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15459382</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2010 May 20;7(5):399-411</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20478541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1102-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17881828</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Aust N Z J Ophthalmol. 1997 May;25(2):177-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9267609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18424797</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Diagn Lab Immunol. 2002 Mar;9(2):378-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11874882</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycologia. 2003 Mar-Apr;95(2):200-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21156606</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2008 Nov 13;456(7219):239-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18923393</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Biol. 2014 Jul;118(7):640-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25088078</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Med Mycol. 2008 Feb;46(1):41-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17885956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Signal Behav. 2010 Mar;5(3):258-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20023385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2009;183(2):291-300</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19496952</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1998 Sep;10(9):1413-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9724689</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2011 May;24(5):543-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21261462</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2003 Mar 1;4(2):119-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20569371</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1442-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12198300</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2004 Apr;198:249-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15199967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2006 Sep 1;43(5):569-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16886148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2010 Dec 10;330(6010):1549-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21148394</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Vaccine Immunol. 2009 Jul;16(7):1047-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19494087</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2006 Jan;1764(1):110-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16249127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004;32(5):1792-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15034147</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2011;12(3):R29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21439036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2006;7:439</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17032440</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Arthritis Rheum. 2003 Dec;33(3):204-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14671729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycoses. 1990 Jun;33(6):317-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2259373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2005 May;11(5):715-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 1987 Feb;25(2):344-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3818928</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2009 Jul;5(7):e1000549</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19578406</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Dermatol. 2014 Feb;25(1):52-e21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24372864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):602-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1804404</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Glycobiology. 2005 Feb;15(2):153-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15385431</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2011 Dec;13(12):1839-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21819515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1995 May 9;92(10):4088-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7753775</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diagn Microbiol Infect Dis. 2013 May;76(1):42-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23537786</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycoses. 2004 Feb;47(1-2):14-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14998394</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2010;11(7):R73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20626842</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12402-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14514891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Sep;42(9):3970-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15364977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Southeast Asian J Trop Med Public Health. 2009 Nov;40(6):1298-305</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20578465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 Nov 1;23(21):2947-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17846036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Jan;40(Database issue):D667-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22064862</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2004 Mar 19;337(2):243-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15003443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2010 Jun 3;465(7298):617-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20520714</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Structure. 1996 Dec 15;4(12):1429-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8994969</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Microbiol. 2009;9 Suppl 1:S3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19278551</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Feb 25;307(5713):1321-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15653466</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Thaïlande</li>
<li>États-Unis</li>
</country>
<region><li>Wisconsin</li>
</region>
</list>
<tree><country name="Thaïlande"><noRegion><name sortKey="Lerksuthirat, Tassanee" sort="Lerksuthirat, Tassanee" uniqKey="Lerksuthirat T" first="Tassanee" last="Lerksuthirat">Tassanee Lerksuthirat</name>
</noRegion>
<name sortKey="Inkomlue, Ruchuros" sort="Inkomlue, Ruchuros" uniqKey="Inkomlue R" first="Ruchuros" last="Inkomlue">Ruchuros Inkomlue</name>
<name sortKey="Khositnithikul, Rommanee" sort="Khositnithikul, Rommanee" uniqKey="Khositnithikul R" first="Rommanee" last="Khositnithikul">Rommanee Khositnithikul</name>
<name sortKey="Krajaejun, Theerapong" sort="Krajaejun, Theerapong" uniqKey="Krajaejun T" first="Theerapong" last="Krajaejun">Theerapong Krajaejun</name>
<name sortKey="Lohnoo, Tassanee" sort="Lohnoo, Tassanee" uniqKey="Lohnoo T" first="Tassanee" last="Lohnoo">Tassanee Lohnoo</name>
<name sortKey="Phaonakrop, Narumon" sort="Phaonakrop, Narumon" uniqKey="Phaonakrop N" first="Narumon" last="Phaonakrop">Narumon Phaonakrop</name>
<name sortKey="Roytrakul, Sittiruk" sort="Roytrakul, Sittiruk" uniqKey="Roytrakul S" first="Sittiruk" last="Roytrakul">Sittiruk Roytrakul</name>
<name sortKey="Rujirawat, Thidarat" sort="Rujirawat, Thidarat" uniqKey="Rujirawat T" first="Thidarat" last="Rujirawat">Thidarat Rujirawat</name>
<name sortKey="Yingyong, Wanta" sort="Yingyong, Wanta" uniqKey="Yingyong W" first="Wanta" last="Yingyong">Wanta Yingyong</name>
</country>
<country name="États-Unis"><region name="Wisconsin"><name sortKey="Sullivan, Thomas D" sort="Sullivan, Thomas D" uniqKey="Sullivan T" first="Thomas D" last="Sullivan">Thomas D. Sullivan</name>
</region>
</country>
</tree>
</affiliations>
</record>

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   |texte=   The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.
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The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.

The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.

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